HERBICIDE-BINDING ACTIVITY OF THE PLASMA MEMBRANE FROM GRASS COLEOPTILES

PLASMA MEMBRANE-RICH FRACTIONS WERE PREPARED FROM MAIZE (ZEA MAYS L.) COLEOPTILES BY LOW SHEAR HOMOGENIZATION AND DIFFERENTIAL AND SUCROSE GRADIENT CENTRIFUGATION. PLASMA MEMBRANE FRAGMENTS WERE IDENTIFIED USING A SPECIFIC CYTOCHEMICAL STAIN. IN A COMPARISON OF 10 DIFFERENT CELL FRACTIONS OF VARYING PLASMA MEMBRANE CONTENT, THE N-L- NAPTHYLPHTHALAMIC ACID (MPA)-BINDING ACTIVITY OF THE FRACTIONS WAS DIRECTLY PROPORTIONAL TO THE CONTENT OF PLASMA MEMBRANE. THE NPA-BINDING APPEARS TO BE STRONG, BUT NON- COVALENT. NPA IS KNOWN TO INHIBIT AUXIN TRANSPORT EFFICIENTLY AND QUICKLY. THUS, THE RESULTS ARE CONSISTENT WITH THE LOCALIZATION OF AUXIN TRANSPORT SITES AT THE PLANT PLASMA MEMBRANE. /AUTHOR/

  • Supplemental Notes:
    • No 7, 17 Pp, 5 FIG, 2 TAB
  • Corporate Authors:

    Purdue University/Indiana Department of Transportation JHRP

    Purdue University, School of Civil Engineering
    West Lafayette, IN  USA  47907-1284
  • Authors:
    • Lembi, C
    • Morre, D J
  • Publication Date: 1971-3

Subject/Index Terms

Filing Info

  • Accession Number: 00204847
  • Record Type: Publication
  • Files: TRIS
  • Created Date: Sep 6 1971 12:00AM