Simultaneous Quantitation of Atenolol, Metoprolol, and Propranolol in Biological Matrices Via LC/MS

Hypertension is a growing medical concern in the United States. With the number of Americans suffering from hypertension increasing, the use of antihypertensives such as beta-blockers is increasing as well. In fact, three beta-blockers - atenolol, metoprolol, and propranolol - were among the 200 most prescribed medications in the United States in 2003. Pilots that successfully manage their hypertension can remain certified to fly. The Federal Aviation Administration currently designates approximately 8% of active pilots as "hypertensive with medication." The Civil Aerospace Medical Institute (CAMI) performs toxicological evaluation on victims of fatal aviation accidents. At CAMI beta-blockers are analyzed using gas chromatography with mass spectrometric detection. The authors have, however, recently developed a liquid chromatography with mass spectrometric detection (LC/MS) method for the simultaneous quantitation of three commonly prescribed beta-blockers - atenolol, metoprolol, and propranolol. One advantage of their LC/MS method is the specificity provided by an ion trap MS. Using an ion trap MS, they were able to conduct MS/MS and MS/MS/MS on each analyte. This method also eliminates the time-consuming and costly derivitization step necessary during GC/MS analysis. Additionally, by utilizing this novel method, any concerns about beta-blocker metabolite and/or sample matrix interference are eliminated. The limits of detection for this method ranged from 0.39 - 0.78 ng/mL, and the linear dynamic range was generally 1.6 - 3200 ng/mL. The extraction efficiencies for each analyte ranged from 58 - 82%. This method was successfully applied to postmortem fluid and tissue specimens obtained from victims of three separate aviation accidents.


  • English

Media Info

  • Media Type: Print
  • Edition: Final Report
  • Features: Figures; References; Tables;
  • Pagination: 21p

Subject/Index Terms

Filing Info

  • Accession Number: 01001422
  • Record Type: Publication
  • Report/Paper Numbers: DOT/FAA/AM-05/10
  • Files: TRIS, USDOT
  • Created Date: Jun 28 2005 4:38PM