The radioimmunoassay for urinary free cortisol described in this paper is simple, rapid, and reproducible. The method uses a commercially available antibody preparation and is performed in two steps. The first step includes an extraction and a column purification to remove materials antigenically similar to cortisol from the urine. The second step is the radioimmunoassay using dextran coated charcoal to separate bound and unbound cortisol. H3-cortisol is added prior to any mechanical manipulation to allow calculation of analytical recovery for the purification procedure. The coefficient of variation for interassay determinations was a maximum of 10.3% and for intra-assay determinations a maximum of 5.7%. Analytical recovery averaged 97.7%. One technician can analyze 100 samples per week. (Author)

  • Supplemental Notes:
    • Presented at the American Public Health Association, 19 Oct 76, Miami Beach, FL.
  • Corporate Authors:

    Naval Health Research Center

    Wright-Patterson AFB, OH  United States  45431
  • Authors:
    • Sokoloff, R L
    • Hilderbrand, R L
  • Publication Date: 1976-9-28

Media Info

  • Pagination: 10 p.

Subject/Index Terms

Filing Info

  • Accession Number: 00167008
  • Record Type: Publication
  • Source Agency: National Technical Information Service
  • Report/Paper Numbers: 76-62, F51524
  • Files: NTIS
  • Created Date: Oct 29 2002 12:00AM