A new solvent compensation mechanism that provides distortion-free spectra for all fluorometric solvents without recalibration when switching solvents is described. The solvent compensator provides sample correction as well as system correction by means of a 2nd light path having its own reference cuvet. A quartz beam-splitter channels 8% of the monochromatic incident energy to the reference cuvet containing the solvent. A photomultiplier electronically compensated to have equal response at all wavelengths monitors the energy transmitted by the solvent. The effects of solvent absorption in both the sample and reference cuvets cancel each other. Examples are given showing comparisons of literature values and absorption spectra to solvent-compensated and noncompensated "corrected" spectra.

  • Corporate Authors:

    Conference on Analytical Chem & Appl Spectroscopy

    Pittsburgh, PA  United States 
  • Authors:
    • Helman, D L
    • Passuater, R A
    • MacEmon, J
  • Publication Date: 1976

Subject/Index Terms

Filing Info

  • Accession Number: 00141597
  • Record Type: Publication
  • Source Agency: Pollution Abstracts
  • Report/Paper Numbers: No. 403
  • Files: TRIS
  • Created Date: Nov 17 1976 12:00AM