MICROBIAL DEGRADATION OF MODEL PETROLEUM AT LOW TEMPERATURES

Two areas of Chesapeake Bay, Colgate Creek in Baltimore Harbor and Eastern Bay, are presently under study, with routine sampling of water and sediment for petroleum- degrading microorganisms (bacteria, yeasts and fungi) by direct plating and enrichment culture. Selected physical and chemical parameters are recorded for each sampling site, and water and sediment samples are extracted for hydrocarbons. Numbers of petroleum-degrading microorganisms were found to correlate with the concentration of benzene- extractable material and were higher for the Colgate Creek than for the Eastern Bay site. Petroleum-degrading microorganisms were isolated from water and sediment at environmental temperatures of o deg, 5 deg, and 10 deg C. A salts medium supplemented with nitrate and phosphate was used to provide optimum conditions for petroleum degradation, whereas, Chesapeake Bay water was used to simulate natural environmental conditions. Higher growth yields and maximum hydrocarbon degradation was observed for microorganisms in the salts medium at 0 deg, 5 deg, and 10 deg C, although significant quantities of hydrocarbons were utilized in some samples grown in Chesapeake Bay water.

  • Corporate Authors:

    Springer Verlag

    175 5th Avenue
    New York, NY  United States  10010
  • Authors:
    • Walker, J D
    • Colwell, R R
  • Publication Date: 1974

Media Info

  • Pagination: p. 63-95
  • Serial:

Subject/Index Terms

Filing Info

  • Accession Number: 00095498
  • Record Type: Publication
  • Source Agency: University of Maryland, College Park
  • Files: TRIS
  • Created Date: May 29 1975 12:00AM