DEVELOPMENT OF A CHEMICAL METHOD FOR DETECTING PETROGENIC HYDROCARBONS IN THE PRESENCE OF BIOGENIC HYDROCARBONS DOWN TO THE 5 PPB LEVEL
The purpose of this project was to develop a chemical method for detecting petrogenic hydrocarbons in the presence of biogenic hydrocarbons at levels below those causing biological harm. The method that was developed involves the use of high pressure liquid chromatography using chloroform as the solvent and measuring fluorescence in the range of 418 nm after excitation at 403 nm. The method is capable of detecting petroleum hydrocarbons in the presence of overwhelming amounts of biological material. It has been demonstrated that the fractions of oil which fluoresce at 418 nm after excitation at 403 nm are among the most persistent fractions in crude oil. Of the six crude oils employed in this investigation, Saudi Arabian was the least responsive to the above technique and yet is still detectable in quantities of less than 0.1 ug and easily quantifiable at 1 ug. Under these circumstances oil is detectable in the ppb range using small to moderate sized water samples (one liter or less). It has been reported (Brown, 1977) that an oil concentration of 5.5 ppb in water would be harmless to the biological community and, therefore, the newly developed method is capable of detecting oil below toxic levels.
- Performed under the technical supervision of the Coast Guard R&D Center, Groton, Connecticut (Report No. CGR&DC 10.79).
Mississippi State University, Mississippi StateMississippi State, MS USA 39762
Department of TransportationOffice of Research and Development, 1200 New Jersey Avenue, SE
Washington, DC USA 20590
- Brown, L R
- Heitz, J R
- Miles, D H
- Minchew, C D
- Publication Date: 1979-2
- Features: References;
- Pagination: 66 p.
- TRT Terms: Detectors; Environmental impacts; Environmental quality; Fluorescence; Monitoring; Oil additives; Oil spill cleanup; Oil spills; Oils
- Old TRIS Terms: Biological effects (Oil); Biological monitoring; Oil content profiles; Oil detection; Oil fluorescence
- Subject Areas: Environment; Marine Transportation;
- Accession Number: 00303108
- Record Type: Publication
- Report/Paper Numbers: CG-D-40-79 Final Rpt.
- Contract Numbers: DOT-CG-81-76-1476
- Files: TRIS, USDOT
- Created Date: Jan 30 1980 12:00AM