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THE ISOLATION AND CHEMICAL AND BIOLOGICAL PROPERTIES OF MICROBIAL EMULSIFYING AGENTS FOR HYDROCARBONS

Emulsifying agents are produced by microorganisms growing on hydrocarbons. Attempts are being made to purify and characterize these molecules in hopes that they may be used as non-toxic, biodegradable emulsifiers which will enhance the rate of oil biodegradation. The factors in the growth medium of Candida petrophilum ATCC 20226 have been partially purified by passage over Amberlite IRC-50 and elution with 50 per cent ethanol. The ethanol fraction has been concentrated and separated into four fractions by thin-layer chromatography on alumina. Two of these fractions have strong emulsification properties, and have been shown to contain more than one component by thin-layer chromatography on cellulose. Preparation of larger amounts of these components is in progress. Preliminary studies on the growth medium of a C. tropicalis strain and Pseudomonas aeruginosa NCIB 9904 have been performed. C. tropicalis produces an emulsifying agent which can be extracted from the medium with hexane. Thin-layer chromatography has shown that there are at least 3 components present, two of which are active agents. The growth medium of Ps. aeruginosa has been evaporated to dryness in vacuo and extracted with hot absolute ethanol. Thin-layer chromatography on silica gel has demonstrated the presence of 4 fractions. Preparation of larger amounts of these fractions will be attempted.

  • Corporate Authors:

    Villanova University

    ,    
  • Authors:
    • Friede, J D
  • Publication Date: 1973-11-28

Media Info

  • Features: References;
  • Pagination: 3 p.

Subject/Index Terms

Filing Info

  • Accession Number: 00050904
  • Record Type: Publication
  • Source Agency: Villanova University
  • Report/Paper Numbers: Rpt #279-1 Annual Rpt
  • Contract Numbers: N00014-73-C-0186
  • Files: TRIS
  • Created Date: Feb 15 1974 12:00AM