AUTOMATIC SIMULTANEOUS DETERMINATION OF LACTATE, PYRUVATE, GLUCOSE AND ETHANOL IN BLOOD

EINE AUTOMATISCHE GLEICHZEITIGE BESTIMMUNG VON LAKTAT, PYRUVAT, GLUCOSE UND AETHANOL IM BLUT AN AUTOMATED SIMULTANEOUS DETERMINATION OF LACTATE, PYRUVATE, GLUCOSE AND ETHANOL IN BLOOD

A METHOD HAS BEEN DEVELOPED FOR THE SIMULTANEOUS DETERMINATION OF LACTATE, PYRUVATE, GLUCOSE AND ETHANOL IN 20 ML OF BLOOD FOR THE AUTOANALYZER. THE BLOOD WAS TREATED BY THE MODIFIED METHOD OF SOMOGYI TO REMOVE ALBUMEN, AND INSTEAD OF NAOH AMMONIAC WAS USED FOR THE NEUTRALIZATION OF THE ZINC SULPHATE SOLUTION. THE METHOD GAVE A SATISFACTORY LEVEL OF REPRODUCIBILITY. WITH STANDARD BLOOD SAMPLES THE PERCENTAGE ERROR FOR LACTATE WAS 0.5%, PYRUVATE 1.0%, THE LACTATE/PYRUVATE RATIO 1.3%, FOR GLUCOSE 1.0% AND ETHANOL 0.7%. WITH BLOOD SAMPLES, WHICH WERE OBTAINED IN TESTS WITH RATS, THE PERCENTAGE ERROR AMOUNTED TO 5.4% FOR LACTATE, 5.2% FOR PYRUVATE, THE LACTATE/PYRUVATE RATIO AMOUNTED TO 5.3%, FOR GLUCOSE 1.3% AND ETHANOL 2.9%.

Language

  • English

Media Info

  • Pagination: 51-7
  • Serial:
    • Blutalkohol
    • Volume: 15
    • Issue Number: 1
    • Publisher: Bund gegen Alkohol im Strassenverkehr eV
    • ISSN: 0006-5250

Subject/Index Terms

Filing Info

  • Accession Number: 01292593
  • Record Type: Publication
  • Source Agency: Bundesanstalt für Straßenwesen (BASt)
  • Files: ITRD
  • Created Date: Nov 21 2010 3:33AM