Use of Alternative Primers for Gender Discrimination in Human Forensic Genotyping

An assay using the Federal Bureau of Investigation’s human Combined DNA Identity System (CODIS) primers has been developed for polymerase chain reaction (PCR)-based human identity testing. Recent forensic literature has identified several human populations that carry a deletion mutation in the Y-chromosome copy of the amelogenin locus. This is the standard locus used for gender determination in CODIS. Additionally, the amelogenin male PCR products are very close in size requiring manual annotation of PCR electrophoresis results for this locus. This study was designed to test several gender-specific primers which are to loci outside the amelogenin region, have well-separated PCR products, and could serve as additions or replacements to amelogenin in our human identity testing assay.

• Record URL:
  http://libraryonline.erau.edu/online-full-text/faa-aviation-medicine-reports/AM08-08.pdf
• 
• Record URL:
  https://rosap.ntl.bts.gov/view/dot/58334
• Corporate Authors:

  Federal Aviation Administration

  Civil Aerospace Medical Institute, P.O. Box 25082
  Oklahoma City, OK  United States  73125

  Federal Aviation Administration

  Office of Aerospace Medicine, 800 Independence Avenue, SW
  Washington, DC  United States  20591
• Authors:
  • Kupfer, Doris M
  • Jenkins, Marita
  • Burian, Dennis
  • Canfield, Dennis V
• Publication Date: 2008-4

Language

• English

Media Info

• Media Type: Print
• Edition: Final Report
• Features: Figures; References; Tables;
• Pagination: 10p

Subject/Index Terms

• TRT Terms: Bioassay; Forensic medicine; Gender; Human beings; Testing
• Uncontrolled Terms: Electrophoresis; Genotyping; Polymerase chain reaction
• Subject Areas: Aviation; Safety and Human Factors; Society;

Filing Info

• Accession Number: 01103345
• Record Type: Publication
• Report/Paper Numbers: DOT/FAA/AM-08/8
• Files: NTL, TRIS, USDOT
• Created Date: Jun 24 2008 7:48AM