RADIOIMMUNOASSAY OF URINARY FREE CORTISOL

The radioimmunoassay for urinary free cortisol described in this paper is simple, rapid, and reproducible. The method uses a commercially available antibody preparation and is performed in two steps. The first step includes an extraction and a column purification to remove materials antigenically similar to cortisol from the urine. The second step is the radioimmunoassay using dextran coated charcoal to separate bound and unbound cortisol. H3-cortisol is added prior to any mechanical manipulation to allow calculation of analytical recovery for the purification procedure. The coefficient of variation for interassay determinations was a maximum of 10.3% and for intra-assay determinations a maximum of 5.7%. Analytical recovery averaged 97.7%. One technician can analyze 100 samples per week. (Author)

• Supplemental Notes:
  • Presented at the American Public Health Association, 19 Oct 76, Miami Beach, FL.
• Corporate Authors:

  Naval Health Research Center

  Wright-Patterson AFB, OH  United States  45431
• Authors:
  • Sokoloff, R L
  • Hilderbrand, R L
• Publication Date: 1976-9-28

Media Info

• Pagination: 10 p.

Subject/Index Terms

• TRT Terms: Absorption; Carbon; Chemical analysis; Solvents; Test procedures; Urine
• Uncontrolled Terms: Antibodies; Cortisol; Solvent extraction
• Old TRIS Terms: Charcoal; Column chromatography; Dextran; Radioimmunoassay
• Subject Areas: Safety and Human Factors;

Filing Info

• Accession Number: 00167008
• Record Type: Publication
• Source Agency: National Technical Information Service
• Report/Paper Numbers: 76-62, F51524
• Files: NTIS
• Created Date: Oct 29 2002 12:00AM