Emulsifying agents are produced by microorganisms growing on hydrocarbons. Attempts are being made to purify and characterize these molecules in hopes that they may be used as non-toxic, biodegradable emulsifiers which will enhance the rate of oil biodegradation. The factors in the growth medium of Candida petrophilum ATCC 20226 have been partially purified by passage over Amberlite IRC-50 and elution with 50 per cent ethanol. The ethanol fraction has been concentrated and separated into four fractions by thin-layer chromatography on alumina. Two of these fractions have strong emulsification properties, and have been shown to contain more than one component by thin-layer chromatography on cellulose. Preparation of larger amounts of these components is in progress. Preliminary studies on the growth medium of a C. tropicalis strain and Pseudomonas aeruginosa NCIB 9904 have been performed. C. tropicalis produces an emulsifying agent which can be extracted from the medium with hexane. Thin-layer chromatography has shown that there are at least 3 components present, two of which are active agents. The growth medium of Ps. aeruginosa has been evaporated to dryness in vacuo and extracted with hot absolute ethanol. Thin-layer chromatography on silica gel has demonstrated the presence of 4 fractions. Preparation of larger amounts of these fractions will be attempted.

  • Corporate Authors:

    Villanova University

  • Authors:
    • Friede, J D
  • Publication Date: 1973-11-28

Media Info

  • Features: References;
  • Pagination: 3 p.

Subject/Index Terms

Filing Info

  • Accession Number: 00050904
  • Record Type: Publication
  • Source Agency: Villanova University
  • Report/Paper Numbers: Rpt #279-1 Annual Rpt
  • Contract Numbers: N00014-73-C-0186
  • Files: TRIS
  • Created Date: Feb 15 1974 12:00AM